Single-particle Cryo-EM of Biological Macromolecules
- Robert M Glaeser, Eva Nogales, Wah Chiu
- May 2021
DescriptionThis edited book is written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM. This is a recently developed method to determine high-resolution structures of biological macromolecules. A major strength is the fact that cryo-EM does not require prior crystallization of protein complexes. Furthermore, it is especially well suited for larger complexes and molecular machines, although it can also be used for macromolecules as small as 40 kDa or less. It also is especially well-suited to deliver high-resolution structural information about multiple conformational states.
- Written for students, postdocs and established investigators who want to enter the field of single-particle cryo-EM.
- Provides a comprehensive, accessible and authoritative introduction to the field of high-resolution structure analysis by single-article cryo-EM.
- Covers all necessary background, ranging from the underlying concepts to practical aspects such as specimen preparation, data-collection, data analysis, and the final validation of results.
Professor Glaeser (UC Berkeley) made major contributions to the initial development of cryo-EM technology, and he currently is active in the improvement of specimen preparation.
Professor Nogales (UC Berkeley) has used cryo-EM throughout her scientific career, successfully applying this technique for the last three decades to systems that defied structural characterization.
Professor Chiu (Stanford University) developed early experimental and computational methods towards 3D structure determination of biomolecules toward atomic resolution.
Table of Contents
1. Introduction and overview
2. Sample preparation
3. Data collection
4. Data processing
5. Map validation
6. Model building and validation
Hardback ISBN: 9780750330374
Ebook ISBN: 9780750330398
Publisher: Institute of Physics Publishing
Series: Biophysical Society-IOP Series